| 质粒名称: | pBabe EGFR Del1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 32062 |
| 存储实验室: | Matthew Meyerson |
|
载体骨架基本信息 |
|
| 载体名称: | pBABEpuro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFR |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3624 bp |
| 基因突变: | Del1(E746-A750) |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SnaB1（克隆过程中销毁） |
| 3' 克隆位点: | SnaB1（克隆过程中销毁） |
| 5' 测序引物 | pBABE 5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBabe EGFR Del1 was a gift from Matthew Meyerson (Addgene plasmid # 32062 ; http://n2t.net/addgene:32062 ; RRID:Addgene_32062) |
| 参考文献部分: | Allele-dependent variation in the relative cellular potency of distinct EGFR inhibitors. Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, Meyerson M. Cancer Biol Ther. 2007 May . 6(5):661-7. 10.4161/cbt.6.5.4003 PubMed 17495523 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBabe EGFR Del1 8710 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBabe EGFR Del1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8710)
AUTHORS Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura
T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK,
Meyerson M
TITLE Allele-dependent variation in the relative cellular potency of
distinct EGFR inhibitors.
JOURNAL Cancer Biol Ther. 2007 May . 6(5):661-7.
PUBMED 17495523
REFERENCE 2 (bases 1 to 8710)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8710
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 120..589
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(810..1409)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(893..913)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1390..1409
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
promoter complement(1419..1748)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1433..1568
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(1487..1506)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 1733..1753
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS complement(5435..5851)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(5451..5467)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(5483..5505)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 6052..6251
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
LTR 6316..6785
/note="long terminal repeat from Moloney murine leukemia
virus"
promoter 6816..6920
/gene="bla"
/label=AmpR promoter
CDS 6921..7781
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7139..7158)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7941..8529
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8430..8449
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8683..8700
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
721 ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg
781 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
841 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
901 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
961 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
1021 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
1081 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
1141 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
1201 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
1261 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
1321 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
1381 aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca
1441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
1501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
1561 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
1621 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
1681 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
1741 tgacacacat tccacagggt cgaccactgt gctggcgaat tcctacatct catgctccaa
1801 taaattcact gctttgtggc gcgaccctta ggtattctgc attttcagct gtggagccct
1861 taaagatgcc atttggcttg gcttccttgg gaaagaagtc ctgctggtag tcagggttgt
1921 ccaggctaat ttggtggctg cctttctggg cccagtgggc agggctgtcg aatgtgctgt
1981 tgacacaggt gggctggaca gtgttgagat actcggggtt gcccactgca gtgctgtggg
2041 ggtcctggta gtgtgggtct ctgctgggcg cggggttcag aggctgattg tgatagacag
2101 gattctgcac agagccagcg ggccttttgg gaacggactg gtttatgtat tcaggcactg
2161 ggaggaaggt gtcgtctatg ctgtcctcag tcaaggcgcc tgtggggtct gagctgtatc
2221 gctgcaagaa gctgtcttcc ttgatgggac agctttgcag cccatttcta tcaatgcaag
2281 ccacggtgga attgttgctg gttgcactca gagagctcag gaggggagtc cgtgacgtgg
2341 aggggctgct gaagaagccc tgctgtggga tgaggtactc gtcggcatcc accacgtcgt
2401 ccatgtcttc ttcatccatc agggcacggt agaagttgga gtctgtagga cttggcaaat
2461 gcattctttc atccccctga atgacaaggt agcgctgggg gtctcgggcc attttggaga
2521 attcgatgat caactcacgg aactttgggc gactatctgc gtctatcatc cagcacttga
2581 ccatgatcat gtagacatcg atggtacata tgggtggctg agggaggcgt tctcctttct
2641 ccaggatgga ggagatctcg ctggcaggga ttccgtcata tggcttggat ccaaaggtca
2701 tcaactccca aacggtcacc ccgtagctcc agacatcact ctggtgggta tagattctgt
2761 gtaaaattga ttccaatgcc atccacttga taggcacttt gcctccttct gcatggtatt
2821 ctttctcttc cgcacccagc agtttggcca gcccaaaatc tgtgatcttg acatgctgcg
2881 gtgttttcac cagtacgttc ctggctgcca ggtcgcggtg caccaagcga cggtcctcca
2941 agtagttcat gccctttgcg atctgcacac accagttgag caggtactgg gagccaatat
3001 tgtctttgtg ttcccggaca tagtccagga ggcagccgaa gggcatgagc tgcgtgatga
3061 gttgcacggt ggaggtgagg cagatgccca gcaggcggca cacgtggggg ttgtccacgc
3121 tggccatcac gtaggcttca tcgaggattt ccttgttggc tttcggagat gttttgatag
3181 cgacgggaat tttaactttc tcaccttctg ggatccagag tcccttatac accgtgccga
3241 acgcaccgga gcccagcact ttgatctttt tgaattcagt ttccttcaag atcctcaaga
3301 gagcttggtt gggagcttct ccactgggtg taagaggctc cacaagctcc ctctcctgca
3361 gcagcctccg cagcgtgcgc ttccgaacga tgtggcgcct tcgcatgaag aggccgatcc
3421 ccagggccac caccagcagc aagaggaggg cccccaccat cccagtggcg atggacggga
3481 tcttaggccc attcgttgga cagccttcaa gacctggccc agtgcatccg taggtgcagt
3541 ttggatggca caggtggcac acatggccgg cgtctgcgta cttccagacc agggtgttgt
3601 tttctcccat gactcctgcc gggcaggtct tgacgcagtg ggggccgtca atgtagtggg
3661 cacactggat acagttgtct ggtccccgtc ctgtgcaggt gatgttcatg gcctgaggca
3721 ggcactctgg gtggcactgt atgcactcag agttctccac aaactccctt ggctcaccct
3781 ccagaaggtt gcacttgtcc acgcattccc tgcctcggct gacattccgg caagagacgc
3841 agtccctggg ctccgggccc cagcagccct cgggggagca caaggcatgg cagacctggc
3901 ctgtggcctt gcagctgttt tcacctctgt tgcttataat tttggttttc tgaccggagg
3961 tcccaaacag ttttttccag tttattgtat ttgcatagca caaatttttg tttcctgaaa
4021 ttatcacatc tccatcactt atctccttga gggagcgtaa tcccaaggat gttatgttca
4081 ggctgacgac tgcaagagaa aactgaccat gttgcttggt cctgccgcgt atgatttcta
4141 ggttctcaaa ggcatggagg tccgtcctgt tttcaggcca agcctgaatc agcaaaaacc
4201 ctgtgatttc ctttacggtt ttcagaatat ccagttcctg tggatccaga ggaggagtat
4261 gtgtgaagga gtcaccccta aatgccaccg gcaggatgtg gagatcgcca ctgatggagg
4321 tgcagttttt gaagtgttta atattcgtag catttatgga gagtgagtct ttaaattcac
4381 caatacctat tccgttacac actttgcggc aaggcccttc gcacttctta cacttgcgga
4441 cgccgtcttc ctccatctca tagctgtcgg ccccacaggc tcggacgcac gagccgtgat
4501 ctgtcaccac ataattacgg ggacacttct tcacgcaggt ggcaccaaag ctgtatttgc
4561 cctcggggtt cacatccatc tggtacgtgg tggggttgta gagcatgagt ggggggcagg
4621 tgtccttgca cgtggcttcg tctcggaatt tgcggcagac caggcagtcg ctctcccggg
4681 ggcctgtgca gcctgcagca cactggttgt ggcagcagtc actgggggac ttgccacggc
4741 agcgcccgga gcactgctgg gcacagatga ttttggtcag tttctggcag ttctcctctc
4801 ctgcacccca gcagctccca ttgggacagc ttggatcaca cttttggcag ctgcccaggt
4861 ggttctggaa gtccatcgac atgttgctga gaaagtcact gctgactatg tcccgccact
4921 ggatgctctc cacgttgcac agggcagggt tgttgctgaa ccgcacggcg ccatgcagga
4981 tttcctgtaa atttctcatg ggcagctcct tcagtccggt tttatttgca tcatagttag
5041 ataagactgc taaggcatag gaattttcgt agtacatatt tcctctgatg atctgcaggt
5101 tttccaaagg aattcgctcc actgtgttga gggcaatgag gacataacca gccacctcct
5161 ggatggtctt taagaaggaa agatcataat tcctctgcac ataggtaatt tccaaattcc
5221 caaggaccac ctcacagtta ttgaacatcc tctggaggct gagaaaatga tcttcaaaag
5281 tgcccaactg cgtgagcttg ttactcgtgc cttggcaaac tttcttttcc tccagagccc
5341 gactcgccgg gcagagcgca gccagcagcg ccaggagcgc tgccccggcc gtcccggagg
5401 gtcgcatgat gtaccaccac actgggatcc ggccggcgcc tagagaagga gtgagggctg
5461 gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg
5521 atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt
5581 caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc
5641 caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc
5701 gcggccatcc gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct
5761 tcttgacatc taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg
5821 tcaaacttaa gggagtggta acagtctggc cctaattttc agacaaatac agaaacacag
5881 tcagacagag acaacacaga acgatgctgc agcgctgcag cagacaagac gcgcggcttc
5941 ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
6001 ctaccagaac cacatatcct gacggggtcg gattccacat cgactccctt cctcaggtcg
6061 ggccacaaaa acggccccca aagtccctgg gacgtctccc agggttgcgg ccgggtgttc
6121 agaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct aactagtacc
6181 gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac agataagttg
6241 ctggccagct tgcctcccgg tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc
6301 ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca gaggagaccc
6361 tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac
6421 gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg gttgtgggct
6481 cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
6541 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
6601 gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga
6661 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
6721 tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata
6781 ttcaggctgc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
6841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
6901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
6961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
7021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
7081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
7141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
7201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
7261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
7321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
7381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
7441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
7501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
7561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
7621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
7681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
7741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
7801 catatatact ttagattgat ttgcggccgc aaaaggatct aggtgaagat cctttttgat
7861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
7921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
7981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
8041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
8101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
8161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
8221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
8281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
8341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
8401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
8461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
8521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
8581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
8641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
8701 gaagcggaag
//
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